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. 2011 May 9;286(26):22806–22813. doi: 10.1074/jbc.M111.246249

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Ouabain treatment (Oua, 10 μmol/liter for 1 h) caused redistribution of the Na/K-ATPase and NHE3 in RPT primary culture of the R rats, but not the S rats. RPTs were isolated from the R and S rats fed with low salt (0.3% NaCl) diet. Cell surface NHE3 and α1 subunit were determined by surface biotinylation (a), and internalized NHE3 and α1 subunit were determined by the protein content in EE fractions (b). n = 4. **, p < 0.01 compared with control. Immunoblotting of integrin-β1 and EEA-1 served as loading control for surface biotinylation and EE fraction, respectively.