TABLE 1.

Initial rates of NHE3 activity in intact mouse proximal tubules (ΔpH/min)

↓, reduced; ↑, increased.

	WT					KO
	n	Untreated	Treated	Change	p	n	Untreated	Treated	Change	p
		ΔpH/min	ΔpH/min	%			ΔpH/min	ΔpH/min	%
Forskolin (10 μm)	6	0.27 ± 0.03	0.16 ± 0.01	↓40 ± 6	<0.01a	6	0.19 ± 0.01	0.11 ± 0.01	↓41 ± 5	<0.001a
A23187 (2.5 μm)	6	0.30 ± 0.04	0.19 ± 0.02	↓38 ± 5	<0.005a	6	0.21 ± 0.02	0.13 ± 0.01	↓36 ± 8	<0.01a
DEX (10 μm)	5	0.29 ± 0.02	0.40 ± 0.03	↑40 ± 7b	<0.005	5	0.21 ± 0.01	0.24 ± 0.01	↑14 ± 5b	<0.05
Basal activity	17	0.29 ± 0.04				17	0.20 ± 0.02			<0.0001

^a Determined using Student's paired t test.

^b Comparing percent increase in NHE3 activity caused by dexamethasone treatment, WT versus Clcn5 KO, p < 0.01.