FIGURE 1.

IRP regulation in response to NO. BMMs derived from Irp1^−/− and Irp2^−/− mice and their corresponding wild-type littermates were exposed to 250 μm DETA/NO for 18 h. Cells were harvested and cytosolic extracts were used to determine IRP1 aconitase activity (mean ± S.D., n = 3) (A) and IRP IRE-binding activity in the presence (B, lower panel) or absence (upper panel) of 2% 2-mercaptoethanol. The experiments were performed at least three times. A representative autoradiogram is shown in B. ***, p < 0.001, Student's t test).