FIGURE 3.

Impact of IRP1 deficiency on NO-mediated regulation of Ft and Fpn. Wild-type and IRP1-null BMMs were exposed to 250 μm DETA/NO for 18 h. A, L-Ft, H-Ft, and Fpn mRNA levels were assayed by real-time quantitative PCR. The histograms display mRNA expression (mean ± S.D., n = 3) as a percentage of untreated wild-type cells after calibration to 18 S ribosomal RNA levels. *, p < 0.05, Student's t test. B, cytosolic levels of L- and H-Ft were analyzed by Western blotting. β-actin was used as a loading control. Fpn expression was analyzed using membrane fractions and vinculin as a loading control. The experiments were performed at least three times, and representative immunoblots are shown.