FIGURE 5.

Transcriptional and IRP1-dependent posttranscriptional regulation of L-Ft and Fpn by NO. Wild-type and IRP1-null BMMs were exposed to 250 μm DETA/NO for 18 h alone or in the presence of the DRB transcriptional inhibitor, as indicated. A, L-Ft and Fpn mRNA levels were assayed by real-time quantitative PCR. The histograms shows mRNA expression (mean ± S.D., n = 3) as a percentage of untreated (no DETA/NO, no DRB) wild-type cells after normalization to 18 S ribosomal RNA levels. B, Western blot analysis of L-Ft (left panels) and Fpn (right panels) levels. Representative immunoblots are shown. β-actin and vinculin, respectively, were used as loading controls. The histograms show L-Ft and Fpn levels (mean ± S.D., n = 3) as a percentage of untreated wild-type cells. *, p < 0.05; **, p < 0.01; ***, p < 0.01; Student's t test.