Skip to main content
. 2011 May 9;286(26):22943–22954. doi: 10.1074/jbc.M110.201194

FIGURE 6.

FIGURE 6.

Cyclin K overexpression leads to increased binding with CDK9 and inhibition of CDK9-Cyclin T1 interaction in Nef-dependent manner. A, Cyclin K binds to CDK9 and inhibits CDK9-Cyclin T1 interaction in pNL4-3-transfected cells. HEK-293T cells were co-transfected with pNL4-3 and CycK expression vector as shown in the figure. 200 μg of lysate was immunoprecipitated (IP) with CDK9 antibody followed by immunoblotting with CycT1, CycK, and CDK9 antibodies. B, Cyclin K inhibits CDK9-Cyclin T1 interaction in a Nef-dependent manner. HEK-293T cells were transfected with pNL4-3 or pNL4-3ΔNef along with pc-CycK. 150 μg of cell lysate was immunoprecipitated with CDK9 antibody followed by immunoblotting with CycT1 antibody. C, Cyclin K binding to CDK9 is increased in the presence of Nef. HEK-293T cells were transfected with pNL4-3 or pNL4-3ΔNef. 150 μg of lysate was incubated with an equal amount of bead-bound GST and GST-CycK protein for a GST pulldown assay as described in the text. The pulldown was detected by immunoblotting with Nef and CDK9 antibodies.