FIGURE 3.

Confocal microscopy for WT hPAR₁E and glycosylation-deficient mutants in KNRK cells. eYFP-tagged PAR₁-expressing KNRK cells were grown on coverslips before fixing and permeabilizing. Cells were stained with propidium iodide prior to visualizing. eYFP is visualized here in green and propidium iodide in red. I, cell surface expression; II, internal receptor expression. Green bar on the right of each panel, 10 μm. A, EV; B, WT hPAR₁E; C, N35QhPAR₁E; D, N62Q/N75QhPAR₁E; E, N250Q/N259QhPAR₁E; F, N35–259QhPAR₁E. The images are representative of four independent experiments.