FIGURE 5.

Cell surface expression and calcium signaling analysis of WT-M and N62Q/N75Q. A, comparison of WT-M and glycosylation-deficient mutant N62Q/N75Q KNRK cell surface expression. Cells at ∼90% confluence were harvested and incubated with the ATAP-2 antibody before incubation with an anti-mouse FITC-conjugated antibody. Cell surface expression was assessed by FACS analysis. Results are expressed as a percentage of the median fluorescence obtained with WT cells. B–D, calcium signaling in the WT-M and glycosylation mutant N62Q/N75Q in response to TFLLR-NH₂, thrombin, and trypsin. Results are expressed as the means ± S.E. of at least four independent experiments.