Skip to main content
. 2011 May 6;286(26):22991–23002. doi: 10.1074/jbc.M110.204271

FIGURE 6.

FIGURE 6.

A, analysis of trypsin disarming in WT-M and N62Q/N75Q. Calcium imaging of Fluo-3-loaded cells was used to assess 100 μm TFLLR-NH2 responses following trypsin and then 5 nm thrombin challenge in WT hPAR1- and hPAR1(N62Q/N75Q)-transfected KNRK cells. The cellular response to TFLLR-NH2 after the addition of thrombin but without pretreatment with trypsin is used as a percentage control. B, analysis of trypsin disarming in N62Q. Shown is hPAR1(N62Q)-transfected KNRK cell calcium signaling response following the addition of 100 nm trypsin, 5 nm thrombin, and 100 μm TFLLR-NH2 and then 2 μm A23187. C, analysis of trypsin disarming in N75Q. Shown is hPAR1(N75Q)-transfected KNRK cell calcium signaling response following the addition of 100 nm trypsin, 5 nm thrombin, 100 μm TFLLR-NH2, and then 2 μm A23187. TRY, trypsin; TH, thrombin; TF, TFLLR-NH2. The data sets are expressed as mean ± S.E. (error bars) from three separate experiments.