FIGURE 8.

Molecular evidence that cathepsin G and proteinase 3 disarm hPAR₁(N62Q/N75Q). CG mutant (A) and proteinase 3 mutant (B) cells loaded with Fluo-3 were stimulated by the addition of either 100 nm cathepsin G or 300 nm proteinase 3 for 2 min and then challenged with 5 nm thrombin, followed by 100 μm TFLLR-NH₂ and then 2 μm calcium ionophore (A23187). TH, thrombin; CG, cathepsin G; PR3, proteinase 3. Results are expressed as the mean ± S.E. (error bars) of at least two independent experiments.