FIGURE 9.

Analysis of hPAR₁(N62Q) and hPAR₁(N75Q) calcium signaling following proteinase stimuli. N62Q (A) and N75Q (B) cells loaded with Fluo-3 were stimulated by the addition of either 50 nm thermolysin, 1.5 nm elastase, 100 nm cathepsin G, or 300 nm proteinase 3 for 2 min and then challenged with 5 nm thrombin, followed by 100 μm TFLLR-NH₂ and then 2 μm A23187. TRY, trypsin; TH, thrombin; TF, TFLLR-NH₂; THE, thermolysin; ELA, elastase; CG, cathepsin G; PR3, proteinase 3. Results are expressed as the mean ± S.E. (error bars) of at least three independent experiments.