TABLE 2.

EC₅₀ values for N-terminal glycosylation-deficient mutant hPAR₁ and their respective matching cell surface expression of WT hPAR₁ agonist concentration effect curves

Cell clones with matched receptor expression assessed by FACS analysis were selected for functional studies. Where it was not possible to match receptor expression between the WT hPAR₁ cell line and a cell line expressing a mutant glycosylation receptor, the WT, WT-H, WT-M, and WT-L with different cell confluence in order to reduce receptor expression were used and thus match the cell surface expression of the mutant cell line. Reduced receptor cell surface expression of the WT-H, WT-M, and WT-L cell lines were confirmed by using FACS analysis.

Cell lines with matched receptor expression	EC50
	TFLLR	Thrombin	Trypsin
	μm	nm	nm
WT-H	17	0.14	33
N35Q	16	0.2	43
WT-L	27	0.2	39
N62Q	29	0.4	43
N75Q	31	0.2	41
WT-M	22	0.12	26
N62Q/N75Q	30	0.3	42