TABLE 1.
Plating efficiency of T7Δ4 on E. coli strains containing plasmids expressing wild-type or genetically altered gp4
The ability of plasmids encoding gp4 variants to support the growth of T7Δ4 in E. coli was examined. The number of plaques formed by plasmids harboring wild-type gp4 is normalized to 1. The relative efficiency of plating obtained with the altered gene 4 constructs was determined by the number of plaques formed by the mutated gene 4 constructs divided by the plaque forming units of wild-type gene 4. The efficiency of plating of ≤10−9 corresponds to the gene 4 construct unable to complement the T7Δ4 growths in the host bacteria.
| pET11b:gp4 construct | Efficiency of plating T7Δ4 |
|---|---|
| Wild-type | 1 |
| E343Q | ≤10−9 |
| E343D | ≤10−9 |
| E343N | ≤10−9 |
| N468A | ≤10−9 |
| R493Q | ≤10−9 |
| R493A/N468A | ≤10−9 |