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Competitive binding data for native YdgR and the V252E mutant. A, inhibition of β-Ala-Lys(AMCA) uptake by alafosfalin. Representative data for the native transporter (black squares) and the V252E mutant (white triangles) from one experiment are shown. B, inhibition of β-Ala-Lys(AMCA) uptake by alanyl-alanine (Ala-Ala). Representative data for the native transporter (black squares) and the V252E mutant (white triangles) are shown. Data for all mutants are summarized in Table 3.
