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ROCK1 undergoes inactivation upon anchorage deprivation. In vivo ROCK1 activity was assayed by monitoring phosphorylation of LIM kinase 1 at Thr508. Logarithmically proliferating REF-Cdc6-RK and REF-Cdc6-aRK cells were cultured in methylcellulose medium for the indicated times, and LIMK1 and LIM kinase phosphorylated at Thr508 (pLIMK1(T508)) were immunodetected. Anc, anchorage.
