TABLE 3.

Expression changes of catabolite regulated genes in GlpK mutant strains

GlpK residue change	glpK (repressed)a		aceE (activated)		aspA (repressed)
	% wt expression S.D.	p valueb	% wt expression S.D.	p value	% wt expression S.D.	p value
V61L	12.71% ± 8.13%	1.4E-04	148.90% ± 43.24%	6.6E-04	15.57% ± 4.55%	1.1E-06
D72V	14.76% ± 12.03%	1.9E-03	117.70% ± 33.06%	0.14	15.50% ± 4.24%	1.0E-06
M271I	30.75% ± 12.50%	3.6E-07	270.13% ± 204.63%	1.8E-04	25.12% ± 23.43%	4.7E-03
Q37P	65.73% ± 22.75%	2.4E-03	290.70% ± 218.60%	6.5E-05	42.48% ± 46.59%	0.12
Correlation to Relative Fitnessc	−0.98	0.003	0.17	(p = 0.78)	−0.99	0.0005

^a Indicates effect of catabolite repression on transcription of gene.

^b Two-tailed one-way ANOVA with Dunnett's post-test were performed on measurements of normalized gene enrichment between wild type and glpK-mutant strains, assuming equal variance. Measured differences in expression that did not meet statistical significance criteria (p < 0.05) have been italicized.

^c Correlation to relative fitness determined by calculating the Pearson coefficient between gene expression and relative strain fitness, with associated two-tailed p values. Correlation coefficients were calculated with wild type as a data point (100% expression).