TABLE 4.
Mutation frequency of various GlpK residues acquired from laboratory evolution of 50 total lineages on glycerol minimal medium
| Residue changea | No. of lineages | |
|---|---|---|
| D72 | A (13), V (7) | 20 |
| M271 | I (3) | 4 |
| A18 | S, T | 2 |
| G94 | D, Gb | 2 |
| G235 | ins. KGG, A | 2 |
| Q37P | 1 | |
| V61L | 1 | |
| Other mutations acquired by only a single strainc | 16 | |
| No glpK mutation | 3 | |
| Multiple glpK mutationsd | 1 |
a Residue change is shown with the locus if the locus was only altered in one lineage; otherwise the resulting residues are shown in the second column. If the locus was altered in multiple lineages, the number of times it was altered to a particular residue is given in parentheses. The residue changes examined in detail in this study are shown in bold.
b One synonymous mutation discovered, G-94-G, which results from nucleotide change g285a.
c Loci of all discovered GlpK mutations in supplemental Table II.
d Three different glpK mutations were detected in one lineage (eBOP86) (supplemental Table S2). This population harbored competing GlpK mutants in different subpopulations, as confirmed by Sanger sequencing of individual colonies.