TABLE 1.
The composition of the cell wall core (mAGP) from M. tuberculosis and M. leprae
| Origin of mAGP | mol % (standard deviation in parenthesesa) normalized to 1 Rha residue (boldface type) |
Macromolecular component ratiosb (propagated standard deviation in parentheses) |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Rha | Arac | Gal | GalNAcd | Total glycosyl residues in AG | GlcNAcd | MurNAcd | DAP | Myc | PGe | AG | Myc | % substitution of AG with Mycf | |
| M. tuberculosis | 0.95 (0.05) | 53.6 (0.92) | 21.3 (0.69) | 0.81 (0.05) | 2.2 (0.46) | 1.91 (0.1) | 7.3 (0.37) | 12.0 (1.0) | 10 | 1.3 (0.03) | 16.4 (2.2) | 79 (9.6) | |
| 1 | 54 | 23 | 0.9 | 79 | 2.3 | 2.0 | 7.7 | 12.7 | |||||
| M. leprae | 1.39 (0.08) | 62 (1.3) | 18 (2.4) | 0.7 (0.2) | 2.0 (0.6) | 1.7 (0.6) | 4.6 (0.4) | 9.4 (1) | 10 | 3.0 (0.23) | 20.8 (4.4) | 42 (2.7) | |
| 1 | 45 | 13 | 0.5 | 59.5 | 1.4 | 1.2 | 3.3 | 6.7 | |||||
a Four separate samples of mAGP from M. tuberculosis and M. leprae were analyzed. The ratios are the average from these four analyses, and the standard deviation is calculated from them. The amount of mAGP from both sources used for analyses was approximately 1 mg.
b These ratios are calculated from the DAP, Rha, and Myc values; see text for justification.
c The total number of Ara residues includes those attached to the mycolic acids and not liberated by acid hydrolysis.
d GlcNAc, GalNH2, and MurNAc cannot be accurately quantitated due to the fact that acid conditions either fail to fully hydrolyze them or stronger conditions degrade them after liberation. Thus, these values are not used for further calculations.
e The PG is chosen to include 10 disaccharide/peptide repeating units for clarity of ratios. There is always one DAP per PG repeating unit.
f Data were calculated based on the ratio of Myc to AG recognizing that 16 mycolic acids per AG is fully mycolylated AG.