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Equilibrium binding of NADP+ (A) or NADPH (B) to wild-type Ct-FDH and its mutants. Insets show Kd values calculated from these experiments. All measurements were done in duplicates. Non-linear curve fitting was performed using Origin 8 (OriginLab). Closed circles, wild-type Ct-FDH; open squares, C707A; open circles, E673A; closed squares, E673Q.
