FIGURE 2.
Overview of the Nurf55-peptide interaction. A, stereo view of polar interactions between Nurf55 and the H4 peptide. Nurf55 and the peptide are depicted as blue and yellow ribbons, respectively. Interacting residues enclosed by a 2Fo − Fc electron density map at 1.0σ are depicted in stick representation. B, schematic representation of the Nurf55-peptide interactions. The three helices and blade 6 forming the binding pocket and blade 7 are depicted in blue, the connecting residues between blades 7 and 6 as a dashed blue line, and the H4 peptide in orange. Hydrophobic interactions are drawn in black, hydrogen bonds in red, and water-mediated hydrogen bonds as dashed red lines. The hydrogen bond network shown in C is indicated with green lines. C, close-up view of the hydrogen bond network between the NH2 group of Arg-39, the carboxamide of Gln-358, and the main chain carbonyls of Asp-362 and Gly-366. The network is indicated with dashed green lines.