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. 2011 May 6;286(26):23407–23418. doi: 10.1074/jbc.M110.214247

FIGURE 3.

FIGURE 3.

PIPP siRNA knockdown in primary hippocampal neurons. A, PIPP siRNA (PIPP) and control (scram) sequence and PIPP proline-rich domains (PRD-1 and PRD-2), 5-phosphatase (5-phos), and SKICH domain (S) are shown. B, lysates of PC12 cells expressing scrambled or PIPP siRNA were immunoblotted with PIPP (PIPP-AB2) or β-tubulin antibodies. C, PIPP expression was standardized to β-tubulin loading by densitometry and expressed relative to scrambled, which was designated as 1. Bars = mean PIPP expression ± S.E. (n = 4; *, p < 0.05). D, quantitative RT-PCR was performed on cDNA synthesized from mRNA extracted from PC12 cells expressing control or PIPP siRNA using GAPDH as a control. PIPP expression was calculated relative to control, which was designated as 1. Bars = mean PIPP expression ± S.E. (n = 4; **, p < 0.01).