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C-terminal acidic residues of Snf1 are not required for regulated Thr210 phosphorylation. A, alignment of sequences lying between the kinase domain (KD) and UBA domain of yeast and human SNF1/AMPKs (residues 305–358 of Snf1). Residues that were changed to Ala in Snf1EA and Snf1EADA are highlighted. B, immunoblot analysis as in Fig. 1. Snf1 proteins were expressed in snf1Δ cells. The sequence encoding Snf1EADA was confirmed for codons 1–500.
