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. 2011 May 12;286(26):23582–23590. doi: 10.1074/jbc.M111.249235

FIGURE 2.

FIGURE 2.

NFIL3 mRNA and protein levels are regulated by IL-10 signaling. A and B, BMDMs (A) and PDMs (B) from C57BL/6 mice (n = 4) were stimulated with LPS or LPS plus IL-10 over time. RNA was collected and analyzed by qRT-PCR for the indicated targets, and the data are presented as the mean ± S.E. C, BMDMs from Stat3+/+;Tie2-cre (WT, n = 2) or Stat3flox/flox;Tie2-cre (n = 2) were stimulated with LPS over time, and NFIL3 mRNA amounts were detected by qRT-PCR. Data are presented as the mean NFIL3 expression relative to GAPDH expression plus the S.D. D, Gut lamina propria and splenic CD11b+ cells were collected. RNA was analyzed by qRT-PCR for the indicated targets, and the data are presented as the mean plus the S.D.