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A NFIL3 binding site is identified in the enhancer region of Il12b. A, sequence of the consensus NFIL3 binding site. B and C, binding of NFIL3 to the enhancer. B, the sequence of gel-shift oligonucleotides used is shown, with each sequential 5- or 6-bp mutation (Mutants A–G). C, IVTT NFIL3 was generated and bound to 32P-labeled oligonucleotide probes and then complexes were resolved by electrophoresis. Binding data are representative of three experiments.
