FIGURE 3.

Accumulation of central domain transcripts in RNA-processing mutants. A, schematic of fission yeast cen1, indicating the central core (cnt), innermost repeat (imr), and outer repeats (otr/dg-dh). Regions amplified by primer pairs (PP1–PP10) used in RT-PCR are indicated below (black bars). B, RT-PCR analysis of transcripts from cen1 and the act1⁺ control. Wild-type, dhp1-1, and pfs2-11 cells were grown at 25 °C and then shifted to 36 °C for 6 h before RNA extraction. Wild-type and dis3-54 cells were grown at 36 °C and shifted to 18 °C for 9 h before RNA extraction. −RT, negative control performed without reverse transcriptase; *, unspecific products. PCR with a chromosomal DNA (chr. DNA) template was included as a positive control. C, RT-PCR analysis of transcripts from cnt2, cnt3, and the act1⁺ control. PP-cnt2 and PP-cnt3 are specific for the central domain of cen2 and cen3, respectively.