(A) Northern blot analysis of adult mouse tissues. U6 small nuclear RNA (snRNA) is the loading control. Brain (Br), heart (Ht), Intestine (Int), liver (Li), lung (Lu), spleen (Spl), and thymus (Thy).
(B) Northern blot analysis of mouse brains from the indicated embryonic (E) and postnatal (P) time points. U6 snRNA is the loading control.
(C) miR-128 LNA in situ hybridization followed by immunofluorescence analysis of the neuronal marker NeuN in a section of adult mouse brain containing the cerebral cortex and hippocampus (low magnification view).
(D, E) miR-128 LNA in situ hybridization followed by immunofluorescence analysis of adult cerebral cortex (D) and hippocampus (E) (both high magnification views). DAPI staining indicates location of cell nuclei (note the distinct glial cell morphologies revealed by GFAP staining).