Table 2.
Components (g/l) | SM1 | SM2 | SM3 | SM4 |
---|---|---|---|---|
K2HPO4 | - | 5.5 | - | - |
KH2PO4 | 1 | 15 | 2 | - |
K2SO4 | - | - | - | 18.2 |
MgSO4.7H2O | 0.5 | 1 | 0.6 | 7.28 |
CaCl2 | 0.1 | - | 5 | - |
NaCl | 0.1 | - | - | - |
CaSO4 | - | - | - | 0.93 |
KOH | - | - | - | 4.4 |
(NH4)2SO4 | - | 4 | 4.5 | - |
Urea | 2 | - | - | - |
Glucose | 20 | 20 | 20 | 20 |
H3PO4 | - | - | - | 26.7 |
Trace elements (mg/l) | a | |||
H3BO3 | 0.5 | - | 8 | 20 |
CuSO4.5H2O | 0.04 | - | 100 | 6000 |
KI | 0.1 | - | 0.9 | 80 |
FeCl3.4H2O | 0.2 | 10 | - | - |
ZnSO4.7H2O | 0.4 | - | 4000 | - |
MnSO4. 2H2O | 0.4 | - | 6500 | 3000 |
EDTA | - | - | 12540 | - |
NaH2PO4.H2O | - | - | 3000 | - |
FeSO4.7H2O | - | - | 2500 | 6500 |
Na2MoO4.2H2O | - | - | 4 | 200 |
CoCl2.6H2O | - | - | 7 | 500 |
NiSO4.7H2O | - | - | 0.8 | - |
ZnCl2 | - | - | - | 20000 |
Vitamins (μg/l) | b | |||
Biotin | 8 | 8 | 80 | |
Thiamin | 200 | 100 | - | |
Myo-inositol | 4 | 2 | - | |
pH | 4.5 | 6 | 5.5 | 5 |
a Concentration in the stock solution. 2 ml/l stock solution were added to SM4 medium
b For SM3 medium, the vitamin solution is composed of 1 mg/l pantothenic acid, 1 mg/l nicotinic acid, 25 mg/l myo-inositol, 1 mg/l thiamin and 1 mg/l pyridoxol hydrochloride, 0.2 mg/l p-amino benzoic acid and 0.05 mg/l biotin. 1.5 ml of this solution were added to 1l of SM3 medium.