Table 2.
Composition of different growth media used in this study
| Components (g/l) | SM1 | SM2 | SM3 | SM4 |
|---|---|---|---|---|
| K2HPO4 | - | 5.5 | - | - |
| KH2PO4 | 1 | 15 | 2 | - |
| K2SO4 | - | - | - | 18.2 |
| MgSO4.7H2O | 0.5 | 1 | 0.6 | 7.28 |
| CaCl2 | 0.1 | - | 5 | - |
| NaCl | 0.1 | - | - | - |
| CaSO4 | - | - | - | 0.93 |
| KOH | - | - | - | 4.4 |
| (NH4)2SO4 | - | 4 | 4.5 | - |
| Urea | 2 | - | - | - |
| Glucose | 20 | 20 | 20 | 20 |
| H3PO4 | - | - | - | 26.7 |
| Trace elements (mg/l) | a | |||
| H3BO3 | 0.5 | - | 8 | 20 |
| CuSO4.5H2O | 0.04 | - | 100 | 6000 |
| KI | 0.1 | - | 0.9 | 80 |
| FeCl3.4H2O | 0.2 | 10 | - | - |
| ZnSO4.7H2O | 0.4 | - | 4000 | - |
| MnSO4. 2H2O | 0.4 | - | 6500 | 3000 |
| EDTA | - | - | 12540 | - |
| NaH2PO4.H2O | - | - | 3000 | - |
| FeSO4.7H2O | - | - | 2500 | 6500 |
| Na2MoO4.2H2O | - | - | 4 | 200 |
| CoCl2.6H2O | - | - | 7 | 500 |
| NiSO4.7H2O | - | - | 0.8 | - |
| ZnCl2 | - | - | - | 20000 |
| Vitamins (μg/l) | b | |||
| Biotin | 8 | 8 | 80 | |
| Thiamin | 200 | 100 | - | |
| Myo-inositol | 4 | 2 | - | |
| pH | 4.5 | 6 | 5.5 | 5 |
a Concentration in the stock solution. 2 ml/l stock solution were added to SM4 medium
b For SM3 medium, the vitamin solution is composed of 1 mg/l pantothenic acid, 1 mg/l nicotinic acid, 25 mg/l myo-inositol, 1 mg/l thiamin and 1 mg/l pyridoxol hydrochloride, 0.2 mg/l p-amino benzoic acid and 0.05 mg/l biotin. 1.5 ml of this solution were added to 1l of SM3 medium.