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Experimental design for SM4 optimization using Modde 6.0 software
| Exp No | Trace element | FeCl3 (mg/l) | Glutamate (g/l) | (NH4)2SO4 (g/l) | Vitamins | hIFNα2b level (μg/l) |
|---|---|---|---|---|---|---|
| 1 | PTM1 | 0 | 0 | 4 | mixture | 2.25 |
| 2 | PTM1 | 10 | 0 | 0 | biotin | 135 |
| 3 | PTM1 | 10 | 1 | 0 | mixture | 216 |
| 4 | SM1 | 10 | 1 | 4 | mixture | 3.37 |
| 5 | SM1 | 0 | 1 | 4 | biotin | 0.25 |
| 6 | SM1 | 10 | 0 | 4 | biotin | 1.68 |
| 7 | SM1 | 0 | 1 | 0 | mixture | 13.5 |
| 8 | PTM1 | 0 | 0 | 0 | biotin | 121.5 |
*SM1: trace elements composition in the synthetic medium 1
