Table 1.
Antibodies and dilutions used in FACS analysis of macrophages
| Primary antibody | Subtype | Dilution | Secondary antibody | Dilution |
|---|---|---|---|---|
| F4/80 (Serotec, Oxford, UK) | IgG | 1:500 | Anti-rat Alexa 488 (Invitrogen) |
1:400 |
| MAC-1 (Serotec, Oxford, UK) | IgG | 1:500 | Anti-rat Alexa-488 (Invitrogen) |
1:400 |
| MR biotinylated (Biolegend, Uithoorn, the Netherlands) | IgG | 1:100 | Streptavidin-Alexa 488 (Invitrogen) |
1:400 |
| LFA-1 produced in house | IgG | 1:250 | Anti-rat Alexa 488 (Invitrogen) |
1:400 |
| CD29 (BD Pharmingen, Breda, the Netherlands) | IgG | 1:100 | Anti-rat Alexa 488 (Invitrogen) |
1:400 |
| CD11a-FITC (eBioscience, Malden, the Netherlands) | IgG | 1:100 | ||