Table 2.

Quantitative methylation-specific PCR primer and TaqMan probe sequences used in the present study

Genes	Forward primer sequence (5'→3')	Probe sequence (5'→3')	Reverse primer sequence (5'→3')
CALCA	GTTTTGGAAGTATGAGGGTGACG	6FAM-ATTCCGCCAATACACAACAACCAATAAACG-TAMRA	TTCCCGCCGCTATAAATCG
CDH1	AATTTTAGGTTAGAGGGTTATCGCGT	6FAM-CGCCCACCCGACCTCGCAT-TAMRA	TCCCCAAAACGAAACTAACGAC
DAPK1	GGATAGTCGGATCGAGTTAACGTC	6FAM-TTCGGTAATTCGTAGCGGTAGGGTTTGG-TAMRA	CCCTCCCAAACGCCGA
IRX2	GCGGGTCGTTTAGGTTAGTATTCG	6FAM-CCCTCCATCCACGCCCGACCGAAA-TAMRA	CGCCGAACAACGAACAAAACG
TIMP3	GCGTCGGAGGTTAAGGTTGTT	6FAM-AACTCGCTCGCCCGCCGAA-TAMRA	CTCTCCAAAATTACCGTACGCG
PAX6	ATATAGGACGGCGGTTTAGGTTG	6FAM-CCCAAAATCCGACCGACTCCAACCCCTA-TAMRA	TTCCGACCGAACGAAAACCTAC
β-Actin	TGGTGATGGAGGAGGTTTAGTAAGT	6FAM-ACCACCACCCAACACACAATAACAAACACA-TAMRA	AACCAATAAAACCTACTCCTCCCTTAA