Figure 3. Barrier properties of the cells cultured in tissue culture inserts in single layers and co-culture was measured by TEER.

Cells were cultured for 1 week at 33°C (A) or a week at 33°C followed by a week at 37°C (B). Bars show mean +/− SEM, n = 20 (mean result of 4 experiments, n = 5 in each). At both temperatures resistance increased when cells in co-culture were compared to cells in a single layer. * p<0.05 by ANOVA with Bonferroni post hoc tests.