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. 2011 Jun 24;6(6):e21203. doi: 10.1371/journal.pone.0021203

Figure 5. Mechanisms of securnine-mediated differentiation.

Figure 5

A. Securinine activates DNA damage signaling. OCI-AML3 cells were treated with securinine (15 µM) for the indicated times and cell lysate was prepared. The lyaste was analyzed for the proteins shown by western blot. B. Inhibition of DNA damage signaling significantly impairs securinine-mediated differentiation. First panel: OCI-AML3 cells were treated with UCN-01 (1 µM), caffeine (1 mM), securinine (15 µM), or a combination for 4 days and the NBT reduction assay was performed. Results are an average of 3 experiments. Second Panel: OCI-AML3 cells stably infected with shp53, shChk1, shp21, or a vector control were treated with securinine (15 µM) for 4 days and the NBT reduction assay and CD11b expression was assessed. C. p21 impairs securinine-mediated growth arrest but not securinine-mediated differentiation. OCI-AML3 cells stably infected with shp21 or vector control were treated with the indicated doses of securinine and were counted after 72 hr using a Coulter Counter.