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. 2011 Jun 24;6(6):e21195. doi: 10.1371/journal.pone.0021195

Table 2. Table showing the dominant 16S rRNA gene ribotypes, explaining the greatest differences/similarities between samples, excised from the DGGE gel.

Band No. Sample type Time period Species ID Group affiliation Close relative (% match)
1 Biofilm (agar slide) 4 h Pseudoalteromonas sp. γ-proteobacteria FM163075 (99%)
2 Biofilm (agar slide) 4 h Pseudoalteromonas sp. γ-proteobacteria DQ665793 (99%)
3 Biofilm (agar slide) 4 h Microbulbifer sp. γ-proteobacteria EF674853 (98%)
4 Biofilm (agar slide) 4 h Pseudoalteromonas sp γ-proteobacteria EU330363 (97%)
5 Biofilm (agar nubbin) 2/4 h Shewanella sp. γ-proteobacteria CP000302 (91%)
6 Biofilm (agar nubbin) 2/4 h β-proteobacterium β-proteobacteria GU257663 (88%)
7 Biofilm (agar nubbin) 2/4 h Pseudoalteromonas sp. γ-proteobacteria GQ849227 (98%)
8 Biofilm (agar nubbin) 2/4 h Vibrio sp. γ-proteobacteria AB519004 (100%)
9 Biofilm (agar nubbin) 2/4 h Klebsiella sp. γ-proteobacteria GQ416635 (90%)
10 Biofilm (agar nubbin) 2/4 h Pseudoalteromonas sp. γ-proteobacteria DQ667134 (100%)
11 Biofilm (agar nubbin) 2/4 h Aeromonas sp. δ-proteobacterium AY689043 (100%)
12 Biofilm (agar nubbin) 2/4 h Prochlorococcus sp. Cyanobacteria GQ272346 (100%)
13 Biofilm (agar nubbin) 2/4 h Shigella sp. γ-proteobacteria FJ193359 (91%)
14 Biofilm (agar nubbin) 2/4 h Enterobacter sp. γ-proteobacteria FN423410 (100%)
15 Biofilm (agar nubbin) 2/4 h Microbulbifer sp. γ-proteobacteria EU837333 (90%)
16 Biofilm (agar nubbin) 24 h Chloroflexi sp. Chloroflexi AB433054 (100%)
17 Biofilm (agar nubbin) 24 h Flavobacteriaceae sp. Flavobacteria EF092242 (100%)
18 Biofilm (agar nubbin) 24 h Thermus sp. Deinococcus-Thermus DQ989458 (96%)
19 Biofilm (agar nubbin) 24 h Pseudoalteromonas sp. γ-proteobacteria FN295786 (100%)
20 Biofilm (agar nubbin) 24 h γ-proteobacterium γ-proteobacteria GU317768 (95%)
21 Biofilm (agar nubbin) 24 h Pseudoalteromonas sp γ-proteobacteria GU229650 (91%)
22 Biofilm (agar nubbin) 24 h Pseudoalteromonas sp. γ-proteobacteria GU726846 (97%)
23 Biofilm (agar nubbin) 24 h Pseudoalteromonas sp. γ-proteobacteria FJ457226 (98%)
24 Biofilm (agar nubbin) 24 h Cyanobacterium Cyanobacteria GU184683 (93%)
25 Biofilm (agar nubbin) 24 h Pseudoalteromonas sp γ-proteobacteria FJ237010 (100%)
26 Biofilm (agar nubbin) 24 h Pseudoalteromonas sp. γ-proteobacteria GU726846 (100%)
27 Biofilm (agar nubbin) 72/96 h Flavobacteria sp. Flavobacteria FN433284 (85%)
28 Biofilm (agar nubbin) 72/96 h Cyanobacterium Cyanobacteria GQ480703 (88%)
29 Biofilm (agar nubbin) 72/96 h Glaciecola sp. γ-proteobacteria EU183316 (95%)
30 Biofilm (agar nubbin) 72/96 h Planctomycetales sp. Planctomycetacia GU084063 (97%)
31 Biofilm (agar nubbin) 72/96 h Aestuariibacter sp. Unknown AB473549 (95%)
32 Biofilm (agar nubbin) 72/96 h Klebsiella sp. γ-proteobacteria GQ416635 (95%)
33 Surface Mucus Layer (Coral) NA Chloroflexi sp. Chloroflexi EU909941 (97%)
34 Surface Mucus Layer (Coral) NA Cyanobacterium Cyanobacteria GQ346809 (100%)
35 Surface Mucus Layer (Coral) NA Cyanobacterium Cyanobacteria FJ967973 (100%)
36 Surface Mucus Layer (Coral) NA Cyanobacterium Cyanobacteria FJ946590 (100%)
37 Surface Mucus Layer (Coral) NA α-proteobacterium α-proteobacteria EF520401 (95%)
38 Surface Mucus Layer (Coral) NA δ-proteobacterium δ-proteobacteria EF188467 (96%)
39 Surface Mucus Layer (Coral) NA Klebsiella sp. γ-proteobacteria GQ471864 (100%)
40 Surface Mucus Layer (Coral) NA Aeromonas sp. δ-proteobacteria EU919223 (100%)
41 Surface Mucus Layer (Coral) NA Burkholderia sp β-proteobacteria EU876657 (100%)
42 Surface Mucus Layer (Coral) NA Aeromonas sp. δ-proteobacteria EU919223 (100%)
43 Surface Mucus Layer (Coral) NA Klebsiella sp. γ-proteobacteria GQ471869 (100%)
44 Surface Mucus Layer (Coral) NA Streptococcus sp. Coccus DQ001071 (97%)
45 Surface Mucus Layer (Coral) NA Klebsiella sp. γ-proteobacteria GQ471864 (100%)
46 Surface Mucus Layer (Coral) NA Trichococcus sp. Coccus EU919224 (87%)
47 Surface Mucus Layer (Coral) NA Shewanella sp. γ-proteobacteria EU919217 (100%)
48 Surface Mucus Layer (Coral) NA Pseudidiomarina sp. γ-proteobacteria FJ887948 (100%)
49 Water Column (Supply) NA Bacteroidetes sp. Bacteroidetes AM238600 (84%)
50 Water Column (Supply) NA Actinobacterium Actinobacteria AY632498 (90%)
51 Water Column (Supply) NA α-proteobacterium α-proteobacteria FJ718457 (96%)
52 Water Column (Supply) NA α-proteobacterium α-proteobacteria GQ350573 (98%)
53 Water Column (Supply) NA α-proteobacterium α-proteobacteria GQ204865 (100%)
54 Water Column (Supply) NA α-proteobacterium α-proteobacteria EF092739 (95%)
55 Water Column (Supply) NA Bacteroidetes sp. Bacteroidetes AB254287 (100%)
56 Water Column (Supply) NA α-proteobacterium α-proteobacteria FJ620860 (95%)
57 Water Column (Supply) NA α-proteobacterium α-proteobacteria EU315614 (97%)
58 Water Column (Supply) NA Flavobacteria sp. Flavobacteria EU600663 (100%)
59 Water Column (Supply) NA Bacteroidetes sp. Bacteroidetes EU315425 (96%)
60 Water Column (Supply) NA Flavobacteriales sp. Flavobacteria AB294989 (100%)
61 Water Column (Supply) NA α-proteobacterium α-proteobacteria FJ532499 (100%)
62 Water Column (Supply) NA Bacteroidetes sp. Bacteroidetes DQ656191 (95%)
63 Water Column (Supply) NA γ-proteobacterium γ-proteobacteria EU315645 (88%)
64 Water Column (Supply) NA γ-proteobacterium γ-proteobacteria GQ257639 (82%)

Representatives from each sample types were included; (Biofilm [agar slides], Biofilm [agar coated artificial nubbins], coral mucus and the water column). Close matches (Blast nt), species identification, group affiliation (identified to closest published relatives on GenBank at the time of comparison) are included within the table. All samples were collected from Heron Island reef flat, March 2009.