Table 2. Table showing the dominant 16S rRNA gene ribotypes, explaining the greatest differences/similarities between samples, excised from the DGGE gel.
Band No. | Sample type | Time period | Species ID | Group affiliation | Close relative (% match) |
1 | Biofilm (agar slide) | 4 h | Pseudoalteromonas sp. | γ-proteobacteria | FM163075 (99%) |
2 | Biofilm (agar slide) | 4 h | Pseudoalteromonas sp. | γ-proteobacteria | DQ665793 (99%) |
3 | Biofilm (agar slide) | 4 h | Microbulbifer sp. | γ-proteobacteria | EF674853 (98%) |
4 | Biofilm (agar slide) | 4 h | Pseudoalteromonas sp | γ-proteobacteria | EU330363 (97%) |
5 | Biofilm (agar nubbin) | 2/4 h | Shewanella sp. | γ-proteobacteria | CP000302 (91%) |
6 | Biofilm (agar nubbin) | 2/4 h | β-proteobacterium | β-proteobacteria | GU257663 (88%) |
7 | Biofilm (agar nubbin) | 2/4 h | Pseudoalteromonas sp. | γ-proteobacteria | GQ849227 (98%) |
8 | Biofilm (agar nubbin) | 2/4 h | Vibrio sp. | γ-proteobacteria | AB519004 (100%) |
9 | Biofilm (agar nubbin) | 2/4 h | Klebsiella sp. | γ-proteobacteria | GQ416635 (90%) |
10 | Biofilm (agar nubbin) | 2/4 h | Pseudoalteromonas sp. | γ-proteobacteria | DQ667134 (100%) |
11 | Biofilm (agar nubbin) | 2/4 h | Aeromonas sp. | δ-proteobacterium | AY689043 (100%) |
12 | Biofilm (agar nubbin) | 2/4 h | Prochlorococcus sp. | Cyanobacteria | GQ272346 (100%) |
13 | Biofilm (agar nubbin) | 2/4 h | Shigella sp. | γ-proteobacteria | FJ193359 (91%) |
14 | Biofilm (agar nubbin) | 2/4 h | Enterobacter sp. | γ-proteobacteria | FN423410 (100%) |
15 | Biofilm (agar nubbin) | 2/4 h | Microbulbifer sp. | γ-proteobacteria | EU837333 (90%) |
16 | Biofilm (agar nubbin) | 24 h | Chloroflexi sp. | Chloroflexi | AB433054 (100%) |
17 | Biofilm (agar nubbin) | 24 h | Flavobacteriaceae sp. | Flavobacteria | EF092242 (100%) |
18 | Biofilm (agar nubbin) | 24 h | Thermus sp. | Deinococcus-Thermus | DQ989458 (96%) |
19 | Biofilm (agar nubbin) | 24 h | Pseudoalteromonas sp. | γ-proteobacteria | FN295786 (100%) |
20 | Biofilm (agar nubbin) | 24 h | γ-proteobacterium | γ-proteobacteria | GU317768 (95%) |
21 | Biofilm (agar nubbin) | 24 h | Pseudoalteromonas sp | γ-proteobacteria | GU229650 (91%) |
22 | Biofilm (agar nubbin) | 24 h | Pseudoalteromonas sp. | γ-proteobacteria | GU726846 (97%) |
23 | Biofilm (agar nubbin) | 24 h | Pseudoalteromonas sp. | γ-proteobacteria | FJ457226 (98%) |
24 | Biofilm (agar nubbin) | 24 h | Cyanobacterium | Cyanobacteria | GU184683 (93%) |
25 | Biofilm (agar nubbin) | 24 h | Pseudoalteromonas sp | γ-proteobacteria | FJ237010 (100%) |
26 | Biofilm (agar nubbin) | 24 h | Pseudoalteromonas sp. | γ-proteobacteria | GU726846 (100%) |
27 | Biofilm (agar nubbin) | 72/96 h | Flavobacteria sp. | Flavobacteria | FN433284 (85%) |
28 | Biofilm (agar nubbin) | 72/96 h | Cyanobacterium | Cyanobacteria | GQ480703 (88%) |
29 | Biofilm (agar nubbin) | 72/96 h | Glaciecola sp. | γ-proteobacteria | EU183316 (95%) |
30 | Biofilm (agar nubbin) | 72/96 h | Planctomycetales sp. | Planctomycetacia | GU084063 (97%) |
31 | Biofilm (agar nubbin) | 72/96 h | Aestuariibacter sp. | Unknown | AB473549 (95%) |
32 | Biofilm (agar nubbin) | 72/96 h | Klebsiella sp. | γ-proteobacteria | GQ416635 (95%) |
33 | Surface Mucus Layer (Coral) | NA | Chloroflexi sp. | Chloroflexi | EU909941 (97%) |
34 | Surface Mucus Layer (Coral) | NA | Cyanobacterium | Cyanobacteria | GQ346809 (100%) |
35 | Surface Mucus Layer (Coral) | NA | Cyanobacterium | Cyanobacteria | FJ967973 (100%) |
36 | Surface Mucus Layer (Coral) | NA | Cyanobacterium | Cyanobacteria | FJ946590 (100%) |
37 | Surface Mucus Layer (Coral) | NA | α-proteobacterium | α-proteobacteria | EF520401 (95%) |
38 | Surface Mucus Layer (Coral) | NA | δ-proteobacterium | δ-proteobacteria | EF188467 (96%) |
39 | Surface Mucus Layer (Coral) | NA | Klebsiella sp. | γ-proteobacteria | GQ471864 (100%) |
40 | Surface Mucus Layer (Coral) | NA | Aeromonas sp. | δ-proteobacteria | EU919223 (100%) |
41 | Surface Mucus Layer (Coral) | NA | Burkholderia sp | β-proteobacteria | EU876657 (100%) |
42 | Surface Mucus Layer (Coral) | NA | Aeromonas sp. | δ-proteobacteria | EU919223 (100%) |
43 | Surface Mucus Layer (Coral) | NA | Klebsiella sp. | γ-proteobacteria | GQ471869 (100%) |
44 | Surface Mucus Layer (Coral) | NA | Streptococcus sp. | Coccus | DQ001071 (97%) |
45 | Surface Mucus Layer (Coral) | NA | Klebsiella sp. | γ-proteobacteria | GQ471864 (100%) |
46 | Surface Mucus Layer (Coral) | NA | Trichococcus sp. | Coccus | EU919224 (87%) |
47 | Surface Mucus Layer (Coral) | NA | Shewanella sp. | γ-proteobacteria | EU919217 (100%) |
48 | Surface Mucus Layer (Coral) | NA | Pseudidiomarina sp. | γ-proteobacteria | FJ887948 (100%) |
49 | Water Column (Supply) | NA | Bacteroidetes sp. | Bacteroidetes | AM238600 (84%) |
50 | Water Column (Supply) | NA | Actinobacterium | Actinobacteria | AY632498 (90%) |
51 | Water Column (Supply) | NA | α-proteobacterium | α-proteobacteria | FJ718457 (96%) |
52 | Water Column (Supply) | NA | α-proteobacterium | α-proteobacteria | GQ350573 (98%) |
53 | Water Column (Supply) | NA | α-proteobacterium | α-proteobacteria | GQ204865 (100%) |
54 | Water Column (Supply) | NA | α-proteobacterium | α-proteobacteria | EF092739 (95%) |
55 | Water Column (Supply) | NA | Bacteroidetes sp. | Bacteroidetes | AB254287 (100%) |
56 | Water Column (Supply) | NA | α-proteobacterium | α-proteobacteria | FJ620860 (95%) |
57 | Water Column (Supply) | NA | α-proteobacterium | α-proteobacteria | EU315614 (97%) |
58 | Water Column (Supply) | NA | Flavobacteria sp. | Flavobacteria | EU600663 (100%) |
59 | Water Column (Supply) | NA | Bacteroidetes sp. | Bacteroidetes | EU315425 (96%) |
60 | Water Column (Supply) | NA | Flavobacteriales sp. | Flavobacteria | AB294989 (100%) |
61 | Water Column (Supply) | NA | α-proteobacterium | α-proteobacteria | FJ532499 (100%) |
62 | Water Column (Supply) | NA | Bacteroidetes sp. | Bacteroidetes | DQ656191 (95%) |
63 | Water Column (Supply) | NA | γ-proteobacterium | γ-proteobacteria | EU315645 (88%) |
64 | Water Column (Supply) | NA | γ-proteobacterium | γ-proteobacteria | GQ257639 (82%) |
Representatives from each sample types were included; (Biofilm [agar slides], Biofilm [agar coated artificial nubbins], coral mucus and the water column). Close matches (Blast nt), species identification, group affiliation (identified to closest published relatives on GenBank at the time of comparison) are included within the table. All samples were collected from Heron Island reef flat, March 2009.