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. 2011 Jun 24;6(6):e21389. doi: 10.1371/journal.pone.0021389

Figure 5. Differentiation expression pattern of ES cells in culture.

Figure 5

GNE+/+ (blue) and GNE−/− (pink) ES cell cultures were analyzed for the RNA expression of several differentiation markers, by RT-PCR: Oct4, as a pluripotent ES marker; neuro-filament medium (NFM), as a neuronal marker; cardiac β-MyHC, as a cardiac cells marker;Pax7, as a skeletal muscle satellite cell marker and MyoD, as a skeletal muscle differentiating marker . β-tubulin was used as a mouse house keeping gene. As a positive control murine muscle C2C12 cells, as well as heart and brain tissues from wild type mouse were used (green panel).