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Enzyme activity in Hepa-1c4 and WT cells and tumours
| nmol/min/mg protein | c4 cells | c4 tumour | WT cells | WT tumour | P value |
|---|---|---|---|---|---|
| LDH | 1640 ± 511 | 861 ± 198 | 2373 ± 421 | 1269 ± 133 | >0.1 |
| PK | 326 ± 4 | 177 ± 40 | 470 ± 15 | 292 ± 12 | <0.05* |
| PFK | 19.1 ± 2.0 | 15.5 ± 1.5 | 20.0 ± 1.5 | 17.8 ± 1.4 | >0.1 |
The activities are expressed as mean ± sem (n = 3) for cultured cells and tumours. The assays were performed under optimal conditions (for details see Methods).* denotes difference between c4 and WT.
