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Exposure to vancomycin induces biosynthesis of the pigmented antibiotics Act and Red in S. coelicolor. This response is markedly reduced in strain M570 that is defective in production of the stringent factor ppGpp. Spore lawns of the indicated strains were spread on SMMS agar plates [81], and paper discs impregnated with vancomycin (or water) were applied to the surface. Cultures were incubated for 3 days at 30°C.
