Table 3.
Minimum inhibitory concentration (MIC) test of mutant strains carrying disruptions in genes targeted based on their transcriptome profiles.
| Strains | Relevant genotype/comments | MIC (μg/ml) | Source/reference | ||
|---|---|---|---|---|---|
| Vancomycin | Bacitracin | Moenomycin | |||
| S. coelicolor A3(2) (M600) | SCP1- SCP2- | ~80 | 40-45 | >300 | Kieser et al. 2000 [81] |
| ΔSCO0600 (H1001) | ΔsigB::apr SCP1- SCP2- | 60-70 | 25-30 | >300 | This study |
| ΔSCO3356 (J2130) | ΔsigE SCP1- SCP2- | 60-70 | 35-40 | >300 | Paget et al. 1999 [20] |
| ΔSCO1513 (M570) | ΔrelA SCP1- SCP2- | 60-70 | 20-25 | >300 | Chakraburtty and Bibb. 1997 [23] |
| ΔSCO3089-3090 (H1003) | ΔSCO3089-3090::apr SCP1- SCP2- | 70-80 | 15-20 | >300 | This study |
| ΔSCO3110-3111 (H1002) | ΔSCO3110-3111::apr SCP1- SCP2- | 70-80 | 30-35 | >300 | This study |
| ΔSCO3089-3090 + ΔSCO3110-3111 (H1004) | ΔSCO3089-3090 + 3110-3111::apr SCP1- SCP2- | 70-80 | <10 | >300 | This study |
| SCO4005::Tn (M900) | SCO4005::Tn SCP1- SCP2- | 70-80 | 25-30 | >300 | Hesketh et al. 2007 [21] |
| SCO5147::Tn (M903) | SCO5147::Tn SCP1- SCP2- | 80-90 | 40-45 | >300 | Hesketh et al. 2007 [21] |
| ΔSCO1875 (H1005) | ΔSCO1875::apr SCP1- SCP2- | <50 | 10-15 | >300 | This study |
| ΔSCO2608 (H1006) | ΔSCO2608::apr SCP1- SCP2- | 60-70 | 40-45 | >300 | This study |
| SCO2897::Tn (H1007) | ΔSCO2897::Tn SCP1- SCP2- | ~60 | 30-35 | >300 | This study |
| SCO3580::Tn (H1008) | ΔSCO3580::Tn SCP1- SCP2- | <50 | 40-45 | >300 | This study |
| SCO3771::Tn (H1009) | ΔSCO3771::Tn SCP1- SCP2- | <50 | 40-45 | >300 | This study |
| ΔSCO3847 (H1010) | ΔSCO3847::apr SCP1- SCP2- | ~60 | 40-45 | >300 | This study |
| SCO3901::Tn (H1011) | ΔSCO3901::Tn SCP1- SCP2- | ~60 | 40-45 | >300 | This study |
| ΔSCO4013 (H1012) | ΔSCO4013::apr SCP1- SCP2- | 70-80 | 40-45 | >300 | This study |
| SCO5039::Tn (H1013) | ΔSCO5039::Tn SCP1- SCP2- | 60-70 | 40-45 | >300 | This study |
| ΔSCO3397 (J3355) | ΔSCO3397::apr SCP1- SCP2- | <50 | 10-15 | >300 | This study |