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. 2011 Jun 24;6(6):e21416. doi: 10.1371/journal.pone.0021416

Figure 4. Effects of the PI3K-AKT and the ERK pathways on regulation of HepG2 cell invasion and MMP-9 expression.

Figure 4

A, Effects of pretreatment of two specific inhibitors, the AKT inhibitor LY294002 (10 µM) and the ERK inhibitor PD98059 (25 µM) for 1 hour on the cell viability after 24-hour incubation assessed by MTT assay. The effects of pretreatment with LY294002 (10 µM) and PD98059 (25 µM) for 1 hour on HepG2 cell invasion (B) and MMP-9 expression (C) after 24-hour incubation. B, Bars are mean±SD from five independent experiments. *Significantly different from DMSO control, P<0.05. C, Bar graphs are derived from densitometric scanning of the blots. Bars are mean±SD from four independent experiments. *Significantly different from DMSO control, P<0.05.