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. 2011 Jun 24;6(6):e21416. doi: 10.1371/journal.pone.0021416

Figure 6. The effect of enhancement of ROS production by berberine (BBR) on its suppressive effects on the activity of PI3K-AKT and ERK pathways, as well as MMP-9 expression and HepG2 cell invasion.

Figure 6

The effect of pretreatment with 10 µM of DPI on berberine-suppressed AKT (A) and ERK (B) activities represented as the levels of phosphorylated forms of AKT and ERK by western blotting after 24-hour incubation. Bar graphs are derived from densitometric scanning of the blots. Bars are mean±SD from three independent experiments. *Significantly different from DMSO control, P<0.05. C, The effect of pretreatment with 10 µM of DPI on berberine-suppressed MMP-9 expression after 24-hour incubation. Bar graphs are derived from densitometric scanning of the blots. Bars are mean±SD from three independent experiments. *Significantly different from DMSO control, P<0.05. D, The effect of pretreatment with 10 µM of DPI on berberine-suppressed HepG2 cell invasion after 24-hour incubation. Bars are mean±SD from five independent experiments. *Significantly different from DMSO control, P<0.05.