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. 2011 Jun 24;6(6):e21482. doi: 10.1371/journal.pone.0021482

Figure 8. The effects of ECSM2 knockdown on bFGF-induced ERK and FAK signaling.

Figure 8

(A) HUVECs were transfected with nonspecific (NS) or ECSM2 siRNAs for 72 h, starved for 8 h, and then stimulated with vehicle (-) or bFGF (10 ng/ml) for 15 min. Protein extracts were analyzed by immunoblotting with anti-pERK, anti-total ERK, anti-β-actin, anti-pFAK(Y397), anti-pFAK(S910), or anti-total FAK, as indicated. (B and C) Statistical analysis of pooled data of pERK and pFAK(Y397) from three independent experiments. Data are mean±SEM. *, P<0.05.