Table 6.
Identification of phenolics and derivatives in 80% methanol and semi-purified ethyl acetate embryo extracts by LC-MS/MSa,d
| Compound | Rt min | MW | MS m/z (+/−) | MS/MS ions m/z (relative intensity) | Ref/std | |
|---|---|---|---|---|---|---|
| Hydroxycinnamic acids | ||||||
| Sinapic acid hexose | 4.9 | 386 | 385 (−) | 223(100) | 179(1) | ref |
| Benzyl alcohol derivatives | ||||||
| Benzyl alcohol hexose pentose | 5.8 | 402 | 401 (−) | 269(100) | 161(28), 239(17), 293(16), 233(10) | ref |
| Flavonoids | ||||||
| (epi)Gallocatechin | 3.5 | 306 | 305 (−) | 179(100) | 261(22), 137(20) | ref |
| Procyanidin dimer (B1 or B2) | 4.7 | 578 | 579 (+) | 427(100) | 409(55), 291(49), 247(20) | ref |
| Catechinb | 6.2 | 290 | 289 (−) | 245(100) | 205(29), 179(16) | std |
| Naringenin pentosec | 10.2 | 404 | 405 (+) | 273(100) | - | |
| (epi)Catechin-(epi)gallocatechin | 11.5 | 594 | 593 (−) | 289(100) | 425(93), 407(48), 245(11) | ref |
| Phloretin hexoside | 14.7 | 436 | 435 (−) | 273(100) | 255(33) | - |
| Naringenin rhamnosidec | 14.2 | 418 | 419 (+) | 273(100) | - | |
| Naringenin | 15.4 | 272 | 271 (−) | 151(100) | std | |
| Naringenin hexoside | 15.5 | 434 | 433 (−) | 271(100) | std | |
| Myricetin rhamno-hexosided,e | 17.6 | 626 | 625 (−) | 316(100) | 317(91), 271(25), 607(9) | ref |
| Naringenin hexosidee | 17.5 | 434 | 433(−) | 271(100) | 151(12) | std |
| Myricetin rhamno-hexosided,e | 18.6 | 626 | 625 (−) | 316(100) | 317(78), 271(20), 287(11), 607(6), 463 (5) | ref |
| Myricetin rhamnosided,e | 19.2 | 464 | 463 (−) | 316(100) | 317 (65), 179(3) | ref |
| Myricetind,e | 19.3 | 317 | 316 (−) | 271(100) | 287(20), 179(13), 151(5) | ref |
| Phloretin hexosidee | 20.5 | 436 | 435 (−) | 273(100) | 167(2) | std |
| Quercetin rhamnosidee | 20.8 | 448 | 447 (−) | 301(100) | 179(2) | ref |
| Phloridzin (phloretin-2-glucoside)e | 21.5 | 436 | 435 (−) | 273(100) | 167(2) | std |
| Stilbenes | ||||||
| Resveratrol derivativec,e | 17.8 | 536 | 535 (−) | 227(100), | 241(43), 307(7) 185(5) | ref |
| Resveratrol derivativec,e | 23.1 | 522 | 521 (−) | 521(100) | 389(80), 227(6) | ref |
a
Rt, retention time; +/− indicates positive ion/negative ion mode; ref/stand., indicate if compared to references/standards.
b
Epicatechin detected at 8.7 min without MS/MS analysis.
c
fragmentation ions indicate compound but not compared to a standard/reference.
d
316/317 indicative derivative of myricetin or myrcetin derivative (Silva et al., 2005)
e
detected in semi-purified ethyl acetate extract