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. 2011 Apr 6;85(1):179–188. doi: 10.1095/biolreprod.110.085555

FIG. 1.

FIG. 1.

Immunoblot of protein tyrosine phosphorylation in stallion (A) and mouse (B) sperm incubated in noncapacitating (NC), capacitating (C), or capacitating conditions plus the soluble adenylyl cyclase (ADCY10) inhibitor KH7. KH7 (60 μM) was used in stallion sperm incubations and 30 μM was used in mouse sperm incubations. KH7 was present in the medium from the time of collection (immediately postejaculation in the stallion or post-swim out in the mouse) and throughout the entire experiment. Experiment repeated at least three times with similar results.