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. 2011 Apr 6;85(1):179–188. doi: 10.1095/biolreprod.110.085555

FIG. 4.

FIG. 4.

Immunofluorescence localization for RAPGEF3 in stallion and mouse sperm. A) RAPGEF3 localizes to the acrosome and equatorial segment in stallion sperm and (B) corresponding bright field image. C) Peptide control demonstrating background levels of fluorescence and (D) corresponding bright field image. E) RAPGEF3 localizes to the acrosome in mouse sperm and (F) corresponding bright field image. G) Pre-immune rabbit IgG control demonstrating background levels of fluorescence and (H) corresponding bright field image. Antibodies and antigenic peptide used were purchased from Santa Cruz Biotechnology as detailed in Materials and Methods. Bar = 5 μm.