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Immunoblot of RAPGEF3 (A) and RAPGEF4 (B) proteins in stallion and mouse sperm. Lanes 1 and 2 are stallion and mouse sperm, respectively, incubated with primary and secondary antibodies. Lanes 3 and 4 are stallion and mouse sperm, respectively, incubated with secondary antibody alone. Antibodies used were purchased from FabGennix International as detailed in Materials and Methods.
