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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2011 May 17;1813(8):1525–1531. doi: 10.1016/j.bbamcr.2011.05.007

Fig. 4.

Fig. 4

KLK7 activates proMMP-9 secreted by MDA-MMP-9 cells. Conditioned medium from MDA-MMP-9 cells was incubated at 37°C with 100 ng of thermolysin-activated KLK7 in the presence of 50 mM EDTA for the indicated times (lanes 1–5). As controls, conditioned medium was incubated without (lane 6) or with thermolysin (lane 7) under similar conditions for 4 hours. As a positive control, 1 pmol of proMMP-9 was incubated with 0.2 pmol thermolysin-activated KLK7 (lane 8) in SFM containing EDTA for 4 hrs. At the end of the incubation period the products were analyzed by gelatin zymography. The truncated MMP-9 (tMMP-9) gelatinolytic products produced by KLK7 are denoted by the arrowheads and thermolysin-directed proteolysis (therm) is indicated by the arrow. Sizes of the protein markers (kDa) are indicated on the left.