TABLE 4.
Shown are some of the genes found by microarray to be differentially expressed (fold-change > 2, p value < 0.05) between the high- and low-frequency segments of the basilar papilla that are ion channels
| Gene symbol | Gene name | Average fold-change (HF/LF) | Corrected p value |
|---|---|---|---|
| CACNA1B | Calcium channel, voltage-dependent, N type, alpha 1B subunit | 1/2.46 | 0.036 |
| CACNA2D1 | Calcium channel, voltage-dependent, alpha 2/delta subunit 1 | 2.96 | 0.028 |
| KCNAB1 | Potassium voltage-gated channel, shaker-related subfamily, beta member 1 | 1/9.46 | 0.0023 |
| KCNAB2 | Potassium voltage-gated channel, shaker-related subfamily, beta member 2 | 1/2.73 | 0.0020 |
| KCND2 | Potassium voltage-gated channel, Shal-related subfamily, member 2 | 1/2.59 | 0.030 |
| KCNF1 | Potassium voltage-gated channel, subfamily F, member 1 | 1/61.46 | 0.0035 |
| KCNG4 | Potassium voltage-gated channel, subfamily G, member 4 | 1/7.53 | 0.0056 |
| KCNJ15 | Potassium inwardly rectifying channel, subfamily J, member 15 | 1/40.82 | 0.0019 |
| 1/26.59 | 0.0012 | ||
| KCNJ2 | Potassium inwardly rectifying channel, subfamily J, member 15 | 1/69.27 | 0.0035 |
| KCNK5 | Potassium channel, subfamily K, member 5 | 1/3.86 | 0.0093 |
| KCNMA1 | Potassium large conductance calcium-activated channel, subfamily M, alpha member 1 | 1/6.82 | 0.025 |
| KCNMB4 | Potassium large conductance calcium-activated channel, subfamily M, beta member 4 | 1/2.27 | 0.0045 |
| SCN2A | Sodium channel, voltage-gated, type II, alpha subunit | 2.33/1 | 0.015 |
| 8.55/1 | 0.0027 | ||
| SCN3B | Sodium channel, voltage-gated, type III, beta | 1/3.58 | 0.020 |
| SCNN1A | Sodium channel, nonvoltage-gated 1 alpha | 2.85/1 | 0.020 |
| 2.62/1 | 0.015 | ||
| TRPC1 | Transient receptor potential cation channel, subfamily C, member 1 | 1/2.77 | 0.035 |
| TRPC3 | Transient receptor potential cation channel, subfamily C, member 3 | 3.55 | 0.022 |
| TRPM3 | Transient receptor potential cation channel, subfamily M, member 3 | 24.24 | 0.0030 |
Multiple values are displayed for the probesets with redundancy. Various sodium, potassium, and calcium channels are all differentially expressed along the tonotopic axis if the chick basilar papilla. Three of the genes in this table were also tested by qPCR (KCNAB1, KCNAB2, SCN2A; see Table 2). Of these three genes, two are also found to be differentially expressed by qPCR (fold-change 95% confidence interval does not include 1)