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HA tagged RUNX2 or RUNX2R131G and Myc tagged CBF-β plasmid were co-transfected in HEK293 cells and proteins were immunoprecipitated with antibodies to HA. Immunoprecipitated proteins or total lysates were separated in 10% SDS-polyacrylamide gels, transferred, and immunoblotted with antibodies to either CBF-β or HA. -: Mock Plasmid; WT: HA tagged Wild Type RUNX2 plasmid; R131G: HA tagged RUNX2R131G plasmid.
