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. Author manuscript; available in PMC: 2011 Oct 15.
Published in final edited form as: Cancer Res. 2010 Oct 5;70(20):8127–8137. doi: 10.1158/0008-5472.CAN-09-4613

Figure 3. Endogenous Twist suppression in MDA-MB-157s decreases Glu expression and organization.

Figure 3

A: Transfection of a non-silencing control (NS ctl) or Twist siRNA (siTwist) in MDA-MB-157s shows suppression of Twist resulted in decreased Glu-tubulin levels. Vimentin and N-cadherin remained unchanged and E-cadherin repression persisted. HMLE-GFP was used as a positive control for E-cadherin expression and β-actin was used as a loading control. B: Following Twist suppression in MDA-MB-157s, nuclear staining of Twist (a,d) is decreased and delocalized compared to the NS Ctl. Twist suppression shows decreased and disrupted Glu (b,e) organization while vimentin (c,f) remained relatively unaffected. C: Live, detached MDA-MB-157s scored blindly following siTwist transfection displayed significantly lower McTN frequencies than NS ctl cells. Columns, mean for six experiments, in which at least 100 CellMask stained cells were counted; bars, SD. (P ≤ 0.05, T-test, black asterisks).