Table 2.
Effect of medium flow on expression of detoxification genesa)
| Gene | Dynamicb) | Static** | Sandwichb) | Collagenb) | FIH |
|---|---|---|---|---|---|
| CYP1A1 | 759.4±550.3 | 29.2±26.4** | 39.7±39.6** | 32±30** | 100±73.9** |
| CYP1A2 | 24.4±8.1 | 2.2±1.4** | 3.8±2.5** | 3.0±3.0** | 100±64.5* |
| CYP2B6 | 230.1±183.1 | 1.8±3.3* | 1.0±0.9* | 1.6±2.4* | 100±53* |
| CYP2C9 | 17.8±7.4 | 4.4±1.9** | 9.0±7.0* | 8.1±3.5* | 100±75.5* |
| CYP2D6 | 29.4±26.5 | 34.1±32.6 | 45.7±25.6 | 33.8±28.4 | 100±29.7** |
| CYP3A4 | 125.8±98.1 | 2.6±1.9* | 1.6±0.7* | 2.3±1.8* | 100±100.1 |
| UGT1A1 | 227.9±118.3 | 55.8±25.8* | 99.6±31.4* | 62.3±33.4* | 100±63.6** |
| UGT2B4 | 96.2±50.5 | 29±17.5* | 77.1±29.4 | 44.5±33.3* | 100±45** |
| UGT2B7 | 26.3±7.6 | 8.1±6.5** | 33.9±26 | 18.3±6.9 | 100±87** |
| GSTA1 | 44.5±16.9 | 14.4±5.7** | 18.9±7.3** | 18.1±7.9** | 100±48.7* |
| NTCP | 1.9±1.4 | 2.4±1.3** | 9.0±2.4** | 14.1±3.6** | 100±71.1** |
| OATP1B3 | 1.9±1.0 | 1.5±1.1 | 4.6±2.8 | 3.1±2.2* | 100±52.8** |
| MDR1 | 284.3±60.3 | 86.9±10.3** | 89.3±32** | 97.9±32.7** | 100±54.1** |
| MRP2 | 1387.5±863.1 | 333.4±271.9* | 344.6±149.8* | 442.6±246.8* | 100±134* |
| AhR | 53.4±19.2 | 26.5±7.6** | 52.4±24.4 | 54.8±42.6 | 100±84.8 |
| ARNT | 34.6±8.3 | 35.5±5.4 | 57.5±11.1** | 42.7±10.4 | 100±51** |
| CAR | 1.9±0.7 | 1.7±1.1 | 3.4±2.7 | 1.8±0.6 | 100±54** |
| PXR | 95.8±21.9 | 86.4±16.9 | 112.0±36.5 | 99.3±29.5 | 100±53.9 |
| GR | 44.9±7.3 | 38.2±11.7 | 45.7±10.6 | 56.8±16.1 | 100±32.7 |
a)
ARNT, AhR nuclear translocator; GR, glucocorticoid receptor; NTCP, Na(+)/taurocholate transport protein; OATP1B3, organic anion transport protein 1B3.
b)
Dynamic and Static: see Fig. 2. Sandwich and Collagen: standard conditions in collagen sandwich configuration or on collagen (0.8 × 106 cells/mL).
See Materials and methods for details.
*
p<0.05.
**
p<0.005 with respect to gene expression in dynamic conditions (n=6).