FIG. 1.

(A) Schematic representation of the rat LHR, the human-TSHR A-subunit (TSHR-289), and the rat-A-subunit LHR equivalent, LHR-289. Included are the restriction sites introduced to clone the LHR and LHR-289 into the shuttle vector, the terminal 6 amino acid residues of the TSHR- and LHR-A-subunits, as well as the 6 putative N-glycans. Because the first N-glycan is not glycosylated in the rat LHR (40), it is depicted with dashed lines (and in a dashed box in panel B). (B) Homologies between the rat- and mouse-LHR ECD and LHR-289. Identical amino acids are indicated by dots; amino acid differences in the mouse LHR are in italics. The signal peptide (first 27 amino acids) is shaded. N-linked glycan motifs are boxed. LHR, luteinizing hormone receptor; TSHR, thyrotropin receptor; ECD, ectodomain.